Mouse Beta-NGF Recombinant Protein (N-His) (MF909012)

Name: Mouse Beta-NGF Recombinant Protein (N-His)
Brand: abinScience
SKU: MF909012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	MF909012
Description	Recombinant Mouse NGF/Beta-NGF Protein, N-His (MF909012) expressed in E. coli, spanning Glu19-Gly241. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P01139
Longueur de protéine	Glu19-Gly241
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Mus musculus (Mouse)
Nature	Recombinant
Cible	Beta-NGF, Beta-nerve growth factor, NGF, NGFB
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	27.37 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Beta-NGF, Beta-nerve growth factor, NGF, NGFB
Contexte	Beta-nerve growth factor (NGF/Beta-NGF) is a ~27 kDa protein. Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. 1. Feng, D. et al. (2010) Journal of molecular biology 396, 967-84. PMID: 20036257 2. Tong, Q. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 3811-21. PMID: 22649032 3. Deinhardt, K. et al. (2011) Science signaling 4, ra82. PMID: 22155786 4. Sun, HL. et al. (2015) Acta crystallographica. Section F, Structural biology communications 71, 906-12. PMID: 26144237
Note	For research use only.