Anti-Mouse NGF/Beta-NGF Recombinant Antibody (MEDI578) (MF909023)

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1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MF909023

Description

Anti-Mouse NGF/Beta-NGF Antibody (MEDI578) [MEDI578] (MF909023) is a rat monoclonal antibody detecting Beta-NGF in ELISA, FCM, RIA. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, FCM, RIA

Host species

Rat

Isotype

IgG2a, kappa

Clone ID

MEDI578

Clonality

Monoclonal

Target

Beta-nerve growth factor, NGFB, Beta-NGF, NGF

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01139

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Beta-nerve growth factor (NGF/Beta-NGF) is a ~27 kDa protein. Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI.

1. Feng, D. et al. (2010) Journal of molecular biology 396, 967-84. PMID: 20036257

2. Tong, Q. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 3811-21. PMID: 22649032

3. Deinhardt, K. et al. (2011) Science signaling 4, ra82. PMID: 22155786

4. Sun, HL. et al. (2015) Acta crystallographica. Section F, Structural biology communications 71, 906-12. PMID: 26144237

Note

For research use only.

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