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Mycobacterium tuberculosis Isocitrate lyase/ICL Recombinant Protein (C-Strep) (JN930022)

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  • 1mg
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Vista general
Número de catálogoJN930022
Descripción
Recombinant Mycobacterium tuberculosis Isocitrate lyase/ICL Protein, C-Strep (JN930022) expressed in B. subtilis. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●High Purity — >90% as determined by SDS-PAGE.
Sistema de expresiónB. subtilis
Número de accesoP9WKK7
Longitud de proteínaMet1-His428
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesMycobacterium tuberculosis
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosEC:4.1.3.1, ICL, Isocitrase, Isocitratase, Isocitrate lyase, icl
Fondo

Isocitrate lyase is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

1. Sharma, V. et al. (2000) Nature structural biology 7, 663-8. PMID: 10932251
2. McKinney, JD. et al. (2000) Nature 406, 735-8. PMID: 10963599
3. Kumar, R. et al. (2008) Proteins 72, 892-900. PMID: 18275086
4. Moynihan, MM. et al. (2014) Biochemistry 53, 178-87. PMID: 24354272
Nota For research use only
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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