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Mycobacterium tuberculosis Isocitrate lyase/ICL Recombinant Protein (C-Strep) (JN930022)

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Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

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  • SDS

Обзор
Каталожный номерJN930022
Описание
Recombinant Mycobacterium tuberculosis Isocitrate lyase/ICL Protein, C-Strep (JN930022) expressed in B. subtilis. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииB. subtilis
Номер доступаP9WKK7
Длина белкаMet1-His428
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыMycobacterium tuberculosis
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEC:4.1.3.1, ICL, Isocitrase, Isocitratase, Isocitrate lyase, icl
Фон

Isocitrate lyase is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

1. Sharma, V. et al. (2000) Nature structural biology 7, 663-8. PMID: 10932251
2. McKinney, JD. et al. (2000) Nature 406, 735-8. PMID: 10963599
3. Kumar, R. et al. (2008) Proteins 72, 892-900. PMID: 18275086
4. Moynihan, MM. et al. (2014) Biochemistry 53, 178-87. PMID: 24354272
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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