Neisseria meningitidis NHBA Recombinant Protein (N-His) (JN891012)

Name: Neisseria meningitidis NHBA Recombinant Protein (N-His)
Brand: abinScience
SKU: JN891012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Даташит
SDS

Обзор

Каталожный номер	JN891012
Описание	Recombinant Neisseria meningitidis NHBA Protein, N-His (JN891012) expressed in E. coli, spanning Met312-Asp427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q9JPP1
Длина белка	Met312-Asp427
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Neisseria meningitidis
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	14.49 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Neisserial heparin binding antigen, NHBA, Genome-derived Neisseria antigen 2132, GNA2132, C2 fragment, nhba, gna2132
Фон	Neisserial heparin binding antigen (NHBA/Dextranase) is a ~44 kDa protein. A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies. Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically. 1. Maritan, M. et al. (2018) PloS one 13, e0201922. PMID: 30133484
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Neisseria meningitidis NHBA Protein, N-His
Western blot

Detected by Anti-NHBA monoclonal antibody (Catalog No: JN891033)

Ссылки

Даташит

Neisseria meningitidis NHBA Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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