Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) (JN891033)

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Overview
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Datasheet

Overview

Catalog No.

JN891033

Description

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) [5H2] (JN891033) is a human monoclonal antibody detecting nhba in ELISA, SPR.

Species reactivity

Neisseria meningitidis

Applications

ELISA, SPR

Host species

Human

Isotype

IgG1, kappa

Clone ID

5H2

Clonality

Monoclonal

Target

nhba, gna2132, Heparin binding protein, Neisserial heparin binding antigen, Genome-derived neisserial antigen 2132, Putative lipoprotein GNA2132, NHBA

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9JPP1

RRID

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) (abinScience Cat# JN891033, RRID:AB_3727794)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Neisserial heparin binding antigen (NHBA/Dextranase) is a ~44 kDa protein. A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies. Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically.

1. Maritan, M. et al. (2018) PloS one 13, e0201922. PMID: 30133484

Note

For research use only.

Images

Bioactivity

Detects Neisseria meningitidis NHBA/Dextranase in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2).
Western blot

Western blot analysis was performed using anti-Neisseria meningitidis NHBA/Dextranase monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant Neisseria meningitidis NHBA (Catalog No: JN891012)
Lane 2: negative control

References

Datasheet

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) (JN891033)

Overview

Images

References

Datasheet

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