Anti-Human Coagulation factor III/Tissue Factor Reference Antibody (Tisotumab, RUO) (HB058016)

Name: Anti-Human Coagulation factor III/Tissue Factor Reference Antibody (Tisotumab, RUO)
Brand: abinScience
SKU: HB058016
Price: 176 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB058016
Description	Tisotumab (HB058016) is a research-grade recombinant antibody targeting CD142. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1-kappa
Système d'expression	Mammalian cells
Applications testées	WB, FCM
Cible	Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P13726
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	HuMax-TF-ADC, TF-011-MMAE, HuMax-TF, 1418628-81-5
Contexte	Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Tisotumab.
Bioactivity

Detects CD142/F3/TF in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Tisotumab
Flow CytoMetry

Flow-cytometry using anti-human CD142 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody (Catalog: HB058016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD142/F3/TF monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD142/F3/TF (Catalog No: HB058011), 50ng
Lane 2: recombinant human CD142/F3/TF (Catalog No: HB058011), 30ng
Lane 3: recombinant human CD142/F3/TF (Catalog No: HB058011), 20ng
Lane 4: recombinant human CD142/F3/TF (Catalog No: HB058011), 10ng
Flow cytometry

Flow-cytometry using APC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.