Rift Valley Fever Virus (RVFV) Research Reagents — Antibodies & Recombinant Proteins

Glycoprotein Gn — Receptor Binding & Neutralization Target
Gn is the attachment glycoprotein that mediates receptor binding (DC-SIGN, heparan sulfate) and is the primary target for virus-neutralizing antibodies. Anti-Gn antibodies block viral entry and are the focus of RVFV therapeutic antibody development. Gn is also the key immunogen in next-generation RVFV subunit vaccines. Available:
• Anti-Gn recombinant antibody SAA2775 (VK101013) — for neutralization studies and diagnostic development
• Recombinant Gn proteins in three formats: C-Fc (VK101011), C-His (VK101021), and N-His (VK101012)
• Anti-Gn polyclonal antibody (VK101014)

Glycoprotein Gc — Class II Fusion Protein
Gc is the class II fusion glycoprotein that undergoes low-pH-triggered conformational change in endosomes to drive membrane fusion. Gc is structurally related to the fusion glycoproteins of other bunyaviruses (CCHFV Gc, hantavirus Gc) and alphaviruses. Anti-Gc antibodies can block post-attachment fusion. Available:
• Recombinant Gc proteins in three formats: C-Fc (VK101031), C-His (VK101041), and N-His (VK101022)
• Anti-Gc polyclonal antibody (VK101024)

Nucleoprotein (NP) — Primary Diagnostic Antigen
NP is the most abundant protein in RVFV-infected cells and the standard target for serological ELISA (IgM/IgG detection) and antigen-capture assays for outbreak diagnosis. NP is highly conserved across RVFV lineages, ensuring broad diagnostic coverage. NP-based DIVA assays can distinguish NSs-deleted vaccine strains from wild-type virus. Available: recombinant NP protein N-His (VK101032) and anti-NP polyclonal antibody (VK101034).

Which reagents are best for RVFV diagnostic ELISA development?
For IgM/IgG serology: use NP protein (VK101032) as ELISA coating antigen — NP elicits early and robust antibody responses and is conserved across all RVFV lineages. For antigen-capture or Gn-based neutralization ELISA: pair anti-Gn mAb SAA2775 (VK101013) as capture with anti-Gn pAb (VK101014) as detection.

Why does RVFV have both Gn and Gc glycoproteins?
Like all bunyaviruses, RVFV uses a two-glycoprotein entry system: Gn handles receptor attachment while Gc drives membrane fusion. This is analogous to CCHFV (Gn + Gc) and hantaviruses (Gn + Gc), but distinct from coronaviruses (single S protein) or paramyxoviruses (H/G + F). Anti-Gn antibodies are typically the most potent neutralizers because they block the initial attachment step. Our panel covers both targets — contact us for combination neutralization assay design.

Can I work with these reagents at BSL-2?
Yes. All RVFV products are recombinant proteins and antibodies — no live or inactivated virus. Standard BSL-1/BSL-2 conditions are sufficient. Live RVFV requires BSL-3+ (BSL-3 Agriculture in the US, BSL-3 in most other countries).

Explore reagents for related bunyaviruses and hemorrhagic fever viruses:

RVFV ✓ CCHFV SFTSV Hantavirus OROV Lassa (LASV) Ebola (EBOV)