Anti-Human CTSB Recombinant Antibody (2A2) (HY436013)

Name: Anti-Human CTSB Recombinant Antibody (2A2)
Brand: abinScience
SKU: HY436013
Price: 110 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY436013

Reactividad de especies

Human

Aplicaciones

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

2A2

Objetivo

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P07858

RRID

Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963)
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Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Guo, R. et al. (2002) Biochemical and biophysical research communications 297, 38-45. PMID: 12220505

2. Ritonja, A. et al. (1985) FEBS letters 181, 169-72. PMID: 3972105

Nota

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human CTSB Antibody (2A2).
Flow CytoMetry

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng