Anti-Human CTSB Recombinant Antibody (2A2) (HY436013)

Name: Anti-Human CTSB Recombinant Antibody (2A2)
Brand: abinScience
SKU: HY436013
Price: 110 USD
Availability: InStock

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20ug
50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY436013

Reactivité des espèces

Human

Applications

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

2A2

Cible

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P07858

RRID

Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Guo, R. et al. (2002) Biochemical and biophysical research communications 297, 38-45. PMID: 12220505

2. Ritonja, A. et al. (1985) FEBS letters 181, 169-72. PMID: 3972105

Note

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Images

SDS-PAGE

SDS-PAGE for Anti-Human CTSB Antibody (2A2).
Flow CytoMetry

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng