Anti-Human CTSB Recombinant Antibody (2A2) (HY436013)

Name: Anti-Human CTSB Recombinant Antibody (2A2)
Brand: abinScience
SKU: HY436013
Price: 110 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HY436013

Реактивность видов

Human

Применения

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a, kappa

ID клона

2A2

Цель

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P07858

RRID

Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963)
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Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Guo, R. et al. (2002) Biochemical and biophysical research communications 297, 38-45. PMID: 12220505

2. Ritonja, A. et al. (1985) FEBS letters 181, 169-72. PMID: 3972105

Примечание

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Изображения

SDS-PAGE

SDS-PAGE for Anti-Human CTSB Antibody (2A2).
Flow CytoMetry

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng