Recombinant Human CD26/DPP4 Protein, C-His (HB015011) expressed in Mammalian Cells, spanning Asn29-Pro766. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
P27487
Longueur de protéine
Asn29-Pro766
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
88.02 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
TP103, Dipeptidyl peptidase IV, ADABP, Dipeptidyl peptidase 4, CD26, DPP4, Dipeptidyl peptidase IV soluble form, Dipeptidyl peptidase IV membrane form, DPP IV, ADCP2, Adenosine deaminase complexing protein 2, ADCP-2, T-cell activation antigen CD26
Contexte
Dipeptidyl peptidase 4 (CD26/DPP4) is a ~88 kDa protein. Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM.
1. Ikushima, H. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 8439-44. PMID: 10900005
2. Durinx, C. et al. (2000) European journal of biochemistry 267, 5608-13. PMID: 10951221
3. Ginés, S. et al. (2002) The Biochemical journal 361, 203-9. PMID: 11772392
4. Ohnuma, K. et al. (2007) The Journal of biological chemistry 282, 10117-10131. PMID: 17287217
5. Aertgeerts, K. et al. (2004) Protein science : a publication of the Protein Society 13, 145-54. PMID: 14691230
9. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD26/DPP4 Protein, C-His
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