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| カタログ番号 | HV511012 |
|---|---|
| 説明 |
Recombinant Human CD288/TLR8 Protein, N-His (HV511012) expressed in E. coli, spanning Glu27-Ile140. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NR97 |
| タンパク質長 | Glu27-Ile140 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.24 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Toll-like receptor 8, CD288, TLR8 |
| 背景 | Toll-like receptor 8 (CD288/TLR8) is a ~119 kDa protein. Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively. 1. Ishii, N. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 5118-28. PMID: 25297876 2. Heinz, LX. et al. (2020) Nature 581, 316-322. PMID: 32433612 3. Greulich, W. et al. (2019) Cell 179, 1264-1275.e13. PMID: 31778653 4. Campbell, GR. et al. (2021) iScience 24, 102295. PMID: 33718825 5. Tanji, H. et al. (2013) Science (New York, N.Y.) 339, 1426-9. PMID: 23520111 6. Tanji, H. et al. (2015) Nature structural & molecular biology 22, 109-15. PMID: 25599397 7. Tanji, H. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 3012-7. PMID: 26929371 8. Qin, J. et al. (2006) The Journal of biological chemistry 281, 21013-21021. PMID: 16737960 9. Doyle, SL. et al. (2007) The Journal of biological chemistry 282, 36953-60. PMID: 17932028 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD288/TLR8 Protein, N-His
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