Anti-Human CD288/TLR8 Polyclonal Antibody (HV511014)

Name: Anti-Human CD288/TLR8 Polyclonal Antibody
Brand: abinScience
SKU: HV511014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV511014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD288/TLR8 (Glu27-Ile140).

Target

Toll-like receptor 8, CD288, TLR8

Purification

Purified by antigen affinity column.

Accession

Q9NR97

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toll-like receptor 8 (CD288/TLR8) is a ~119 kDa protein. Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively.

1. Ishii, N. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 5118-28. PMID: 25297876

2. Heinz, LX. et al. (2020) Nature 581, 316-322. PMID: 32433612

3. Greulich, W. et al. (2019) Cell 179, 1264-1275.e13. PMID: 31778653

4. Campbell, GR. et al. (2021) iScience 24, 102295. PMID: 33718825

5. Tanji, H. et al. (2013) Science (New York, N.Y.) 339, 1426-9. PMID: 23520111

6. Tanji, H. et al. (2015) Nature structural & molecular biology 22, 109-15. PMID: 25599397

7. Tanji, H. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 3012-7. PMID: 26929371

8. Qin, J. et al. (2006) The Journal of biological chemistry 281, 21013-21021. PMID: 16737960

9. Doyle, SL. et al. (2007) The Journal of biological chemistry 282, 36953-60. PMID: 17932028

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CD288/TLR8 polyclonal antibody at 1ug/mL on recombinant Human CD288/TLR8 (Catalog No. HV511012).

References

Datasheet