Recombinant Human CD316/IGSF8 Protein, C-His (HV379011) expressed in Mammalian Cells, spanning Met1-Thr579. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии
Mammalian cells
Номер доступа
Q969P0
Длина белка
Met1-Thr579
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
62.38 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
EWI2, IGSF8, CD316, Glu-Trp-Ile EWI motif-containing protein 2, Keratinocytes-associated transmembrane protein 4, LIR-D1, Prostaglandin regulatory-like protein, CD81P3, EWI-2, KCT-4, CD81 partner 3, Immunoglobulin superfamily member 8, IgSF8, KCT4, PGRL
Фон
Immunoglobulin superfamily member 8 (CD316/IGSF8) is a ~65 kDa protein. Member of the immunoglobulin superfamily (IgSF) that links tetraspanin-enriched microdomains to the actin cytoskeleton and plays several important roles in innate and adaptive immunity. Acts as an inducible receptor of HSPA8 on dendritic cells to enhance the CCL21/SLC-dependent migration of activated mature dendritic cells while attenuating their antigen-specific stimulatory capacities. In complex with alpha-actinins ACTN1 and ACTN4, regulates actin dynamics in the immune synapse and subsequent T-cell activation. Inhibits the entry of several viruses such as hepatitis C Virus (HCV) or HIV-1. Mechanistically, promotes a change in CD81 organization at the plasma membrane by significantly restricting its diffusion which in turn influences CD81 interaction with Claudin-1/CLDN1, preventing CLDN1 from acting as a co-receptor required for HCV entry.
1. Stipp, CS. et al. (2001) The Journal of biological chemistry 276, 40545-54. PMID: 11504738
2. Stipp, CS. et al. (2003) The Journal of cell biology 163, 1167-77. PMID: 14662754
3. Kettner, S. et al. (2007) Molecular and cellular biology 27, 7718-26. PMID: 17785435
4. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882
5. Potel, J. et al. (2013) Cellular microbiology 15, 1234-52. PMID: 23351194
6. Whitaker, EE. et al. (2019) Viruses 11. PMID: 31757023
7. Li, Y. et al. (2024) Cell 187, 2703-2716.e23. PMID: 38657602
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human CD316/IGSF8 Protein, C-His
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