ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
31.61 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
CD316, CD81 partner 3, CD81P3, EWI-2, EWI2, Glu-Trp-Ile EWI motif-containing protein 2, IGSF8, IgSF8, Immunoglobulin superfamily member 8, KCT-4, KCT4, Keratinocytes-associated transmembrane protein 4, LIR-D1, PGRL, Prostaglandin regulatory-like protein
Background
Immunoglobulin superfamily member 8 is a ~65 kDa protein. Member of the immunoglobulin superfamily (IgSF) that links tetraspanin-enriched microdomains to the actin cytoskeleton and plays several important roles in innate and adaptive immunity. Acts as an inducible receptor of HSPA8 on dendritic cells to enhance the CCL21/SLC-dependent migration of activated mature dendritic cells while attenuating their antigen-specific stimulatory capacities. In complex with alpha-actinins ACTN1 and ACTN4, regulates actin dynamics in the immune synapse and subsequent T-cell activation. Inhibits the entry of several viruses such as hepatitis C Virus (HCV) or HIV-1. Mechanistically, promotes a change in CD81 organization at the plasma membrane by significantly restricting its diffusion which in turn influences CD81 interaction with Claudin-1/CLDN1, preventing CLDN1 from acting as a co-receptor required for HCV entry.
1. Stipp, CS. et al. (2001) The Journal of biological chemistry 276, 40545-54. PMID: 11504738
2. Stipp, CS. et al. (2003) The Journal of cell biology 163, 1167-77. PMID: 14662754
3. Kettner, S. et al. (2007) Molecular and cellular biology 27, 7718-26. PMID: 17785435
4. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882
5. Potel, J. et al. (2013) Cellular microbiology 15, 1234-52. PMID: 23351194
6. Whitaker, EE. et al. (2019) Viruses 11. PMID: 31757023
7. Li, Y. et al. (2024) Cell 187, 2703-2716.e23. PMID: 38657602
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