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Human IL-31 Recombinant Protein (C-His) (HV678011)

Human IL-31 Recombinant Protein (C-His)
Human IL-31 Recombinant Protein (C-His)
Human IL-31 Recombinant Protein (C-His)
Human IL-31 Recombinant Protein (C-His)
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Spécification :
  • 50ug
  • 100ug
  • 1mg
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  • Reférences

  • Fiche technique

  • SDS

Aperçu
Numéro de catalogueHV678011
Description
Recombinant Human IL31 Protein, C-His (HV678011) expressed in Mammalian Cells, spanning Leu22-Thr164. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — C-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionMammalian cells
Numéro d'accèsQ6EBC2
Longueur de protéineLeu22-Thr164
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsIL-31, Interleukin-31, IL31
Contexte

Interleukin-31 (IL31) is a ~18 kDa protein. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.

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1. Dillon, SR. et al. (2004) Nature immunology 5, 752-60. PMID: 15184896
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Note For research use only.
Images
  • Human IL-31 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human IL31 Protein, C-His

  • Human IL-31 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Anti-Human IL31 (BMS-981164) (Cat No.: HV678016) in indirect ELISA.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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