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Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Human VEGF165 Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHB941011
Описание
Recombinant Human VEGFA/VEGF165 Protein, C-His (HB941011) expressed in Mammalian Cells, spanning Met1-Arg191.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииMammalian cells
Номер доступаP15692-4
Длина белкаMet1-Arg191
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Проверенные приложения WB
Цель VEGF165, VEGF-165, L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 23.28 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияVEGF165, VEGF-165, L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor
Примечание For research use only.
Изображения
  • Human VEGF165 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human VEGFA/VEGF165 Protein, C-His

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects VEGFA in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects BD0801 in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects Dilpacimab in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects Conbercept in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects Human VEGFA Antibody in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detects Vanucizumab in indirect ELISAs.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Ivonescimab in indirect ELISA.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Faricimab (Cat No.: HT073016) in indirect ELISA.

  • Human VEGF165 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-VEGFA/VEGF165 monoclonal antibody (Catalog No: HB941056)

  • Human VEGF165 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-VEGFA/VEGF165 monoclonal antibody (Catalog No: MY392307)

  • Human VEGF165 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-VEGFA/VEGF165 monoclonal antibody (Catalog No: MB941013)

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Detected by SAA0902 (Catalog No: HB941033) in indirect ELISA.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Immobilized HW338021 Human VEGFR2 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB941011 Human VEGFA/VEGF165 Recombinant Protein (C-His), the EC 50 is 60.1 ng/mL.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Immobilized HW338031 Human VEGFR2 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB941011 Human VEGFA/VEGF165 Recombinant Protein (C-His), the EC 50 is 36.2 ng/mL.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Immobilized HB265011 Human FLT1 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB941011 Human VEGFA/VEGF165 Recombinant Protein (C-His), the EC 50 is 101 ng/mL.

  • Human VEGF165 Recombinant Protein (C-His)

    Bioactivity

    Immobilized HB265021 Human FLT1/VEGFR1 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB941011 Human VEGFA/VEGF165 Recombinant Protein (C-His), the EC 50 is 16.0 ng/mL.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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