







| Catalog No. | HB941056 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Host species | Humanized |
| Isotype | IgG1-kappa-[scFv]2 |
| Expression system | Mammalian Cells |
| Clonality | Monoclonal |
| Target | Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A, Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279 |
| Endotoxin level | Please contact the lab for this information. |
| Purity | >95% purity as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P15692 & Q15116 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| Alternate Names | TetravalentBispecific, AK112, AK-112, AK1122428381-53-5 |
| Background | Ivonescimab (AK112) is a PD-1/VEGF Bispecific Antibody. Ivonescimab can be used for cancer research. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |

Detects VEGF165 in indirect ELISA.

Detects recombinant human PD1 (Cat No.: HS870021) in indirect ELISA.

Flow-cytometry using FITC anti-human VEGFA&PD1 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA&PD1 monoclonal antibody (Catalog: HB941056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control
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