Anti-Mouse VEGFA Recombinant Antibody (G6) (MB941013)

Name: Anti-Mouse VEGFA Recombinant Antibody (G6)
Brand: abinScience
SKU: MB941013
Price: 110 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MB941013

Species reactivity

Human, Mouse, Rat, Rabbit

Applications

Blocking, ELISA, FCM

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

Tested applications

WB, FCM

Target

L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q00731

RRID

Anti-Mouse VEGFA Antibody (G6) (abinScience Cat# MB941013, RRID:AB_3727197)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vascular endothelial growth factor A, long form (VEGFA) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA is the therapeutic target of bevacizumab (Avastin).

1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

Bioactivity

Detects Mouse VEGFA in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Mouse VEGFA Antibody (G6)
Flow CytoMetry

Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control

References

Datasheet

Anti-Mouse VEGFA Recombinant Antibody (G6).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse VEGFA Recombinant Antibody (G6) (MB941013)

Overview

Images

References

Datasheet

SDS

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