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| カタログ番号 | MV974011 |
|---|---|
| 説明 |
Recombinant Mouse CD274/PD-L1/B7-H1 Protein, C-His (MV974011) expressed in Mammalian Cells, spanning Met1-His239. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | Q9EP73 |
| タンパク質長 | Met1-His239 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| テスト済みアプリケーション | WB |
| ターゲット | B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 27.87 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1 |
| 背景 | Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). _x005F_x000D_ _x005F_x000D_1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124 _x005F_x000D_
_x005F_x000D_
2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 _x005F_x000D_
_x005F_x000D_
3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480 _x005F_x000D_ |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Mouse CD274/PD-L1/B7-H1 Protein, C-His
Detects Mouse CD274/PD-L1/B7-H1 in indirect ELISAs.
Detected by Anti-CD274/PD-L1/B7-H1 monoclonal antibody (Catalog No: HB769023)
Detected by anti-mouse PDL1 antibody (Catalog No: MV974040) in indirect ELISA
Detected by Iv0226 (Catalog No: MB651030) in indirect ELISA.
Detected by C3 (Catalog No: MV974033) in indirect ELISA.
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