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Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359), FITC (MV974217)

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Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359), FITC

Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359), FITC

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Overview

Catalog No.	MV974217
Description	Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), FITC [SAA0359] (MV974217) is a mouse monoclonal antibody detecting PD-L1 in ELISA, FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Mouse
Applications	ELISA, FCM
Host species	Mouse
Isotype	VHH, mFc
Clone ID	SAA0359
Conjugation	FITC
Clonality	Monoclonal
Target	Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Endotoxin level	Please contact with the lab for this information.
Accession	Q9EP73
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). 1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124 2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: MV974217, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359), FITC.pdf

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Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359), FITC