Border Disease Virus (BDV) Research Reagents — Antibodies & Recombinant Proteins

Envelope Glycoprotein E2 — Neutralization & Vaccine Target
E2 is the immunodominant surface glycoprotein and the primary target for virus-neutralizing antibodies. E2 forms a heterodimer with E1 and mediates receptor binding (CD46 in some pestiviruses). E2-based subunit vaccines and DIVA diagnostics (E2 marker vaccines) are the standard strategy for pestivirus control. Available:
• E2 C-Fc (VK750041) — Fc-tagged for Protein A capture, SPR binding, and oriented ELISA coating
• E2 C-His (VK054011) — for direct plate coating and pull-down assays
• E2 N-His (VK750022) — alternative tag orientation for epitope exposure
• Anti-E2 polyclonal antibody (VK750024)

Erns (E0) — Secreted RNase Glycoprotein
Erns is a unique pestivirus protein with no homolog in other virus families — a secreted glycoprotein possessing intrinsic RNase T2 activity that degrades extracellular dsRNA to suppress type I/III interferon induction. Erns is detectable in serum of PI animals and serves as a diagnostic antigen for identifying persistent BDV infection. Erns is also a target for antiviral strategies: RNase-dead Erns mutants restore innate immune sensing and attenuate virus replication. Available:
• Erns recombinant protein N-His (VK054012)
• Anti-Erns polyclonal antibody (VK054014)

Do BDV reagents cross-react with BVDV and CSFV?
BDV, BVDV, and CSFV are closely related pestiviruses with E2 amino acid identity of ~60–70%. Cross-reactivity varies by antibody epitope — conserved epitopes may cross-react, while hypervariable regions are species-specific. Our anti-BDV E2 pAb is raised against BDV-specific E2 but may show partial BVDV cross-reactivity. For confirmed CSFV-specific reagents, see our CSFV page. Contact us for cross-reactivity data.

What is the difference between Erns and E2 for BDV diagnostics?
E2-based ELISA detects anti-E2 antibodies (seroconversion marker, post-vaccination or infection). Erns-based assays detect the Erns protein itself in serum — Erns is actively secreted by infected cells and is detectable in PI animals that may be seronegative. Using both targets together (E2 serology + Erns antigen detection) provides the most comprehensive BDV diagnostic coverage.

Which E2 protein format is best for serology ELISA development?
E2 C-Fc (VK750041) is recommended for oriented capture on Protein A-coated plates — the Fc tag ensures consistent antigen orientation and maximizes antibody binding sites exposure. For direct coating, E2 C-His (VK054011) is a simpler option with reliable adsorption.

Explore reagents for related pestiviruses and veterinary viruses:

BDV ✓ CSFV PRRSV ASFV PRV NDV