Porcine Reproductive & Respiratory Syndrome Virus (PRRSV) Research Reagents — Antibodies & Recombinant Proteins

Glycoprotein 5 (GP5) — Major Neutralization Target
GP5 is the major envelope glycoprotein and the primary target for neutralizing antibodies. GP5 forms a heterodimer with M protein and contains the immunodominant neutralizing epitope (ectodomain B epitope). GP5 sequence hypervariability drives immune evasion and complicates vaccine design. Available: recombinant GP5 protein N-GST&C-His (VK016012) and anti-GP5 polyclonal antibody (VK016014).

Minor Glycoproteins GP2, GP3, GP4 — Receptor Binding Complex
GP2, GP3, and GP4 form a heterotrimer that mediates receptor binding (CD163, sialoadhesin/CD169). GP4 is the primary receptor-binding subunit and an increasingly important vaccine target. Available:
• GP4: C-His (VK406011), C-Fc (VK406021), N-His (VK406012), and anti-GP4 pAb (VK406014)
• GP3: C-Fc (VK508011), N-His (VK508012), and anti-GP3 pAb (VK508014)
• GP2: C-Fc (VK459011), N-His (VK459012), and anti-GP2 pAb (VK459014)

Nucleocapsid Protein (N/NP) — Diagnostic Antigen
NP is the most abundant viral protein and the standard target for PRRSV ELISA diagnostics (IDEXX PRRS X3, indirect ELISA). NP is highly conserved between PRRSV-1 and PRRSV-2, enabling broad diagnostic coverage. Available: recombinant NP protein N-His (VK752012), recombinant anti-NP antibodies Nb1 (VK752013) and SAA2530 (VK752023), and anti-NP polyclonal antibody (VK752014).

NSP4 / 3CL Protease
NSP4 is the main viral protease (chymotrypsin-like 3CL protease) responsible for polyprotein processing. NSP4 is a primary target for antiviral drug development — analogous to SARS-CoV-2 Mpro targeted by nirmatrelvir. Available: recombinant NSP4/3CL protein N-His (VK417012) and anti-NSP4 polyclonal antibody (VK417024).

NSP7 — Immunomodulatory Protein
NSP7 is involved in viral replication and has immunomodulatory functions. Available: recombinant NSP7 protein N-His (VK417022) and anti-NSP7 polyclonal antibody (VK417014).

NSP9 / RdRp (RNA Polymerase)
NSP9 is the RNA-dependent RNA polymerase — the core enzyme of the replication/transcription complex and a target for polymerase inhibitors (remdesivir analogs). Available: anti-NSP9/RdRp nanobody Nb8 (VK534013) for detection and functional studies.

Do your reagents cover both PRRSV-1 and PRRSV-2?
Our NP protein and anti-NP antibodies are broadly reactive across both PRRSV species due to high NP sequence conservation. Glycoprotein reagents (GP2–GP5) are derived from PRRSV-2 sequences and may have reduced cross-reactivity with PRRSV-1 strains. Contact us for PRRSV-1-specific reagent availability or custom development from your isolate sequence.

Which proteins are best for PRRSV DIVA diagnostics?
DIVA (Differentiating Infected from Vaccinated Animals) strategies typically use NP-based ELISA for infection detection (NP antibodies appear early and persist) alongside GP5 or GP2/GP3/GP4-based assays for strain-specific characterization. Our GP2/GP3/GP4 proteins in C-Fc format are ideal for oriented ELISA coating in differential serological assays.

Can the NSP4/3CL protease be used for antiviral drug screening?
Yes. Our recombinant NSP4/3CL protein (VK417012) is suitable for in vitro protease activity assays (fluorogenic/chromogenic substrate cleavage) and high-throughput inhibitor screening. The PRRSV 3CL protease is structurally related to coronavirus Mpro, enabling cross-application of assay formats.

Explore reagents for related swine and veterinary viruses:

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