Human Nectin-2/CD112 Recombinant Protein (C-His) (HV456011)

Name: Human Nectin-2/CD112 Recombinant Protein (C-His)
Brand: abinScience
SKU: HV456011
Price: 298 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HV456011
Description	Recombinant Human CD112/Nectin-2/PVRL2 Protein, C-His (HV456011) expressed in Mammalian Cells, spanning Met1-Leu360. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	Mammalian cells
Numéro d'accès	Q92692
Longueur de protéine	Met1-Leu360
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Applications testées	WB
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	39.79 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2, Poliovirus receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2
Contexte	Nectin-2 (CD112/PVRL2) is a ~57 kDa protein. Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. 1. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705 2. Warner, MS. et al. (1998) Virology 246, 179-89. PMID: 9657005
Note	For research use only.

Images

Bioactivity

Detects Human CD112/Nectin-2/PVRL2 Antibody (SAA0405) in indirect ELISAs.
Western blot

Detected by Anti-CD112/Nectin-2/PVRL2 monoclonal antibody (Catalog No: HV456107)
Bioactivity

Detected by Research Grade Anti-Human CD112/Nectin-2/PVRL2 Antibody (NTX-1107C) (Catalog No: HP700046) in indirect ELISA
Bioactivity

Detected by Anti-Human CD112/Nectin-2/PVRL2 Antibody (SAA2115) (Catalog No: HV456207) in indirect ELISA
Bioactivity

Detected by SAA2116 (Catalog No: HV456023) in indirect ELISA
Bioactivity

Detected by SAA2117 (Catalog No: HV456033) in indirect ELISA
Bioactivity

Detected by SAA2364 (Catalog No: HV456013) in indirect ELISA
Bioactivity

Immobilized HV456011 Human CD112/Nectin-2/PVRL2 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HS737021 Human CD226/DNAM-1 Recombinant Protein (C-Fc), the EC 50 is 28.8 μg/mL.