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Human CD277/BTN3A1 Recombinant Protein (C-Fc) (HT139011)

Human CD277/BTN3A1 Recombinant Protein (C-Fc)
Human CD277/BTN3A1 Recombinant Protein (C-Fc)
Human CD277/BTN3A1 Recombinant Protein (C-Fc)
Human CD277/BTN3A1 Recombinant Protein (C-Fc)
Human CD277/BTN3A1 Recombinant Protein (C-Fc)
Human CD277/BTN3A1 Recombinant Protein (C-Fc)
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  • 概要

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  • 参考文献

  • データシート

  • SDS

概要
カタログ番号HT139011
説明
Recombinant Human CD277/BTN3A1 Protein, C-Fc (HT139011) expressed in Mammalian Cells, spanning Met1-Gly254. Purity: >90% by SDS-PAGE.
Highlights
  • ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムMammalian cells
アクセッション番号O00481
タンパク質長Met1-Gly254
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 53.87 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277
背景

Butyrophilin subfamily 3 member A1 (CD277/BTN3A1) is a ~57 kDa protein. Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.

注意事項 For research use only.
イメージ
  • Human CD277/BTN3A1 Recombinant Protein (C-Fc)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CD277/BTN3A1 Protein, C-Fc

  • Human CD277/BTN3A1 Recombinant Protein (C-Fc)

    Bioactivity

    Detects Human CD277/BTN3A1 Antibody (103.2#) in indirect ELISAs.

  • Human CD277/BTN3A1 Recombinant Protein (C-Fc)

    Western blot

    Detected by Anti-CD277/BTN3A1 monoclonal antibody (Catalog No: HT139107)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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