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Anti-Human CD277/BTN3A1 Antibody (103.2#) (HT139107)

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  • Datasheet

Overview
Catalog No.HT139107
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone ID103.2#
Clonality Monoclonal
Target BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession O00481
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternate Nameshu103.2
Note For research use only.
Images
  • Bioactivity

    Detects CD277/BTN3A1 in indirect ELISAs.

  • Flow-cytometry

    Flow-cytometry using anti-human CD277 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD277 antibody monoclonal antibody (Catalog # HT139107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human CD277/BTN3A1 Antibody (103.2#)

  • Western blot

    Western blot analysis was performed using anti-CD277/BTN3A1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human CD277/BTN3A1 (Catalog No: HT139011)
    Lane 2: negative control

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