中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HF004326 | ||||
|---|---|---|---|---|---|
| 설명 |
Anti-Human EGFR Reference Antibody (ASKB 1202, RUO) (HF004326) is a monoclonal antibody detecting EGFR.
|
||||
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, FCM, Functional assay | ||||
| 클론성 | Monoclonal | ||||
| 발현 시스템 | Mammalian cells | ||||
| 성질 | Recombinant | ||||
| 타겟 | EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 | ||||
| 내독소 수준 | < 10 EU/mg as determined by LAL method. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | P00533 | ||||
| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||
| 제품 사용 정보 |
| ||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 배경 | Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix). _x005F_x000D_ _x005F_x000D_1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725 _x005F_x000D_
_x005F_x000D_
2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536 _x005F_x000D_
_x005F_x000D_
3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960 _x005F_x000D_
_x005F_x000D_
4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 _x005F_x000D_
_x005F_x000D_
5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049 _x005F_x000D_
_x005F_x000D_
6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079 _x005F_x000D_
_x005F_x000D_
7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 _x005F_x000D_
_x005F_x000D_
8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704 _x005F_x000D_ | ||||
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
문의하기
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
