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| 카탈로그 번호 | HY276053 |
|---|---|
| 설명 |
Anti-Human alpha-Fetoprotein/AFP-L3 Recombinant Antibody (SRR0101) (HY276053) is a rabbit monoclonal antibody detecting alpha-Fetoprotein/AFP-L3 in IF, FCM, WB. Suitable for Human.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | IF, FCM, WB |
| 숙주 종 | Rabbit |
| 클론성 | Monoclonal |
| 이소타입 | IgG |
| 클론 ID | SRR0101 |
| 테스트된 응용 분야 | IF, FCM, WB |
| 타겟 | AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, HPAFP |
| 내독소 수준 | < 10 EU/mg as determined by LAL method. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P02771 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 배경 | alpha-Fetoprotein/AFP-L3 is a protein. |
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-alpha-Fetoprotein/AFP-L3 monoclonal antibody at 1 ug/mL on various samples.
Lane 1: HepG2 cell lysate
HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting alpha-Fetoprotein/AFP-L3 (HY276053) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of alpha-Fetoprotein/AFP-L3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow-cytometry using anti-Human AFP/AFP-L3 antibody. Permeabilized HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human AFP/AFP-L3 monoclonal antibody (Catalog HY276053, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.
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