Anti-ACTG1 Polyclonal Antibody (HX058014)

Name: Anti-ACTG1 Polyclonal Antibody
Brand: abinScience
SKU: HX058014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HX058014

설명

Anti-ACTG1 Polyclonal Antibody (HX058014) is a rabbit polyclonal antibody detecting ACTG1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Absidia glauca.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ACTG1 (Met1-Phe375).

타겟

ACTG, Gamma-actin, ACTG1, Actin, cytoplasmic 2

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P60710, P60709, P60711, P63260, P63259, P63261, P68136, P68133, P68033, P68032, P68134, P62736, P63269, P68035, P63268, P62738, P63267, P62737, Q8BFZ3, Q6S8J3, Q562R1, P0CG38, P0CG39, A5A3E0, Q9BYX7

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Actin, cytoplasmic 1 (ACTG1) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built.

1. Baarlink, C. et al. (2013) Science (New York, N.Y.) 340, 864-7. PMID: 23558171

2. Plessner, M. et al. (2015) The Journal of biological chemistry 290, 11209-16. PMID: 25759381

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-ACTG1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human ACTG1 (Catalog No: HX058012)
Lane 2: PC12 cell lysate
Lane 3: MCF7 cell lysate

참고 문헌

데이터시트

Anti-ACTG1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ACTG1 Polyclonal Antibody (HX058014)

개요

이미지

참고 문헌

데이터시트

SDS

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