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| 카탈로그 번호 | HP522014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human ADD3 (Met1-Lys227). | ||||||||
| 타겟 | Adducin-like protein 70, ADDL, ADD3, Gamma-adducin | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q9UEY8 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Gamma-adducin (ADD3) is a ~79 kDa protein. Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin (Probable). Involved in myogenic reactivity of the renal afferent arteriole (Af-art), renal interlobular arteries and middle cerebral artery (MCA) to increased perfusion pressure. Involved in regulation of potassium channels in the vascular smooth muscle cells (VSMCs) of the Af-art and MCA ex vivo. 1. Kruer, MC. et al. (2013) Annals of neurology 74, 805-14. PMID: 23836506 | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ADD3 (HP522014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ADD3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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