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| 카탈로그 번호 | HY519014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human ALDOC (Asp34-Ser281). | ||||||||
| 타겟 | Brain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P09972 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Fructose-bisphosphate aldolase C (ALDOC) is a ~39 kDa protein. Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis. | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (HY519014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: LO2 cell lysate
Lane 3: k562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Mouse brain lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse muscle lysate
Lane 8: Mouse heart lysate
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