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Anti-AOAH Polyclonal Antibody (HB957014)

Anti-AOAH Polyclonal Antibody
Anti-AOAH Polyclonal Antibody
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사양:
  • 20ug
  • 50ug
  • 100ug
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

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개요
카탈로그 번호HB957014
설명
Anti-AOAH Polyclonal Antibody (HB957014) is a rabbit polyclonal antibody detecting AOAH in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human AOAH (Ser24-Gln300).
타겟 AOAH, Acyloxyacyl hydrolase
정제 Purified by antigen affinity column.
등록번호 P28039
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Acyloxyacyl hydrolase (AOAH) is a ~65 kDa protein. Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

1. Hagen, FS. et al. (1991) Biochemistry 30, 8415-23. PMID: 1883828
2. Gorelik, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E896-E905. PMID: 29343645
3. Staab, JF. et al. (1994) The Journal of biological chemistry 269, 23736-42. PMID: 8089145
참고사항 For research use only.
이미지
  • Anti-AOAH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AOAH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: BxPC-3 cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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