Anti-APLN Polyclonal Antibody (HV404014)

Name: Anti-APLN Polyclonal Antibody
Brand: abinScience
SKU: HV404014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HV404014

설명

Anti-APLN Polyclonal Antibody (HV404014) is a rabbit polyclonal antibody detecting APLN in ELISA, IHC, WB. Suitable for Human, Bovine, Rattus norvegicus, and Mus musculus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human APLN (Ser24-Phe77).

타겟

APEL, Apelin, APJ endogenous ligand, APLN

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q9ULZ1, Q9TUI9, Q9R0R3, Q9R0R4

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Apelin (APLN) is a ~8 kDa protein. Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions.

1. Habata, Y. et al. (1999) Biochimica et biophysica acta 1452, 25-35. PMID: 10525157

2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587

3. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871

4. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-APLN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-APLN Polyclonal Antibody (HV404014)

개요

이미지

참고 문헌

데이터시트

SDS

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