Anti-ARL8B Polyclonal Antibody (HC083014)

Name: Anti-ARL8B Polyclonal Antibody
Brand: abinScience
SKU: HC083014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HC083014

설명

Anti-ARL8B Polyclonal Antibody (HC083014) is a rabbit polyclonal antibody detecting ARL8B in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Bovine, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ARL8B (Met1-Ser186).

타겟

ADP-ribosylation factor-like protein 8B, EC:3.6.5.2, ADP-ribosylation factor-like protein 10C, Novel small G protein indispensable for equal chromosome segregation 1, ARL8B, ARL10C, GIE1

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q9NVJ2, Q66HA6, Q9CQW2, Q2KI07, Q6NZW8, Q5ZKQ8, Q96BM9, Q8VEH3, Q9VHV5

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

ADP-ribosylation factor-like protein 8B is a ~21 kDa protein. Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis.

1. Okai, T. et al. (2004) Journal of cell science 117, 4705-15. PMID: 15331635

2. Hofmann, I. et al. (2006) Journal of cell science 119, 1494-503. PMID: 16537643

3. Bagshaw, RD. et al. (2006) Biochemical and biophysical research communications 344, 1186-91. PMID: 16650381

4. Pu, J. et al. (2015) Developmental cell 33, 176-88. PMID: 25898167

5. Deshar, R. et al. (2016) The FEBS journal 283, 4583-4599. PMID: 27808481

6. Marwaha, R. et al. (2017) The Journal of cell biology 216, 1051-1070. PMID: 28325809

7. Rosa-Ferreira, C. et al. (2011) Developmental cell 21, 1171-8. PMID: 22172677

8. Tuli, A. et al. (2013) Molecular biology of the cell 24, 3721-35. PMID: 24088571

9. Michelet, X. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 3160-3169. PMID: 29592961

10. Garg, S. et al. (2011) Immunity 35, 182-93. PMID: 21802320

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-ARF1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: DU145 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Lncap cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse spleen lysate
Lane 9: Mouse lung lysate
Lane 10: Rat brain lysate

참고 문헌

데이터시트

Anti-ARL8B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ARL8B Polyclonal Antibody (HC083014)

개요

이미지

참고 문헌

데이터시트

SDS