Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014)

Name: Anti-ASFV UBC/pI215L Polyclonal Antibody
Brand: abinScience
SKU: VK475014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

VK475014

설명

Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014) is a rabbit polyclonal antibody detecting pI215L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

African swine fever virus (ASFV)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant ASFV UBC/pI215L (Met1-Thr200).

타겟

Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L

정제

Purified by antigen affinity column.

등록번호

P27949

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Ubiquitin-conjugating enzyme E2 (UBC/pI215L) is a ~24 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication. May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery. Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling.

1. Freitas, FB. et al. (2018) Scientific reports 8, 3471. PMID: 29472632

2. Barrado-Gil, L. et al. (2020) Frontiers in microbiology 11, 622907. PMID: 33384682

3. Barrado-Gil, L. et al. (2021) Viruses 13. PMID: 34204411

4. Huang, L. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 2754-2769. PMID: 34759016

5. Hingamp, PM. et al. (1995) Journal of virology 69, 1785-93. PMID: 7853518

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-ASFV UBC/pI215L Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014)

개요

이미지

참고 문헌

데이터시트

SDS

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