Anti-ATG4B Polyclonal Antibody (HB598014)

Name: Anti-ATG4B Polyclonal Antibody
Brand: abinScience
SKU: HB598014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HB598014

설명

Anti-ATG4B Polyclonal Antibody (HB598014) is a rabbit polyclonal antibody detecting Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant human ATG4B (Met1-Leu393).

타겟

Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q9Y4P1, A0A0G2QC33, Q8BGE6, Q6PZ03

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cysteine protease ATG4B is a ~44 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

2. Tanida, I. et al. (2004) The Journal of biological chemistry 279, 36268-76. PMID: 15187094

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Satoo, K. et al. (2009) The EMBO journal 28, 1341-50. PMID: 19322194

5. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Yang, Z. et al. (2015) The Journal of biological chemistry 290, 26549-61. PMID: 26378241

8. Jo, YK. et al. (2016) Oncotarget 7, 57186-57196. PMID: 27527864

9. Li, Y. et al. (2017) Autophagy 13, 1145-1160. PMID: 28633005

10. Pengo, N. et al. (2017) Nature communications 8, 294. PMID: 28821708

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-ATG4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ATG4B Polyclonal Antibody (HB598014)

개요

이미지

참고 문헌

데이터시트

SDS

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