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Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H) (JN898083)

Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
가격(USD):
사양:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
수량:
문의하기
  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호JN898083
종 반응성Bacillus anthracis
응용 분야ELISA, Neutralization, WB
숙주 종Mouse
클론성Monoclonal
이소타입IgG2a
클론 ID83K2H
타겟 pagA, Protective antigen, PA, Anthrax toxins translocating protein, PA-83, Bacillus anthracis
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P13423
RRID

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (83K2H) (abinScience Cat# JN898083, RRID:AB_3729003)
_x005F_x005F_x000D_

형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Protective antigen (PA/pagA) is a ~85 kDa protein. Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.

1. Bradley, KA. et al. (2001) Nature 414, 225-9. PMID: 11700562
2. Bradley, KA. et al. (2003) The Journal of biological chemistry 278, 49342-7. PMID: 14507921
3. Santelli, E. et al. (2004) Nature 430, 905-8. PMID: 15243628
4. Lacy, DB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13147-51. PMID: 15326297
5. Singh, Y. et al. (1991) The Journal of biological chemistry 266, 15493-7. PMID: 1651334
6. Mock, M. et al. (2001) Annual review of microbiology 55, 647-71. PMID: 11544370
참고사항 For research use only.
이미지
  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)

    SDS-PAGE

    SDS-PAGE for Anti-Bacillus anthracis PA/pagA/PA-83 Recombinant Antibody (83K2H)

  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)

    Bioactivity

    Detects recombinant Bacillus anthracis PA/pagA/PA-83 (Catalog No: JN898022) in indirect ELISA

  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)

    Western blot

    Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Bacillus anthracis PA/pagA/PA-83(Catalog No: JN898022)
    Lane 2: negative control
    Lane 3: negative control

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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