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Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014)

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개요
카탈로그 번호JN872014
설명
Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014) is a rabbit polyclonal antibody detecting BliGO in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Bacillus licheniformis
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Bacillus licheniformis BliGO/thiO (Met1-Arg369).
타겟 Glycine oxidase, GO, 1.4.3.19, BliGO, thiO
정제 Purified by antigen affinity column.
등록번호 S5FMM4
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Glycine oxidase (BliGO/thiO) is a ~40 kDa protein. Catalyzes the FAD-dependent oxidative deamination of glycine, leading to glyoxylate, ammonia and hydrogen peroxide. Is also able to act on various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine), and thus may be involved in the degradation pathway that allows B.licheniformis J33-8 to grow with glyphosate as the sole source of carbon. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

1. Zhang, K. et al. (2016) Enzyme and microbial technology 85, 12-8. PMID: 26920475
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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