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| 카탈로그 번호 | HV095014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-CACYBP Polyclonal Antibody (HV095014) is a rabbit polyclonal antibody detecting CacyBP in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
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| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human CACYBP (Ala2-Phe228). | ||||||||
| 타겟 | S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q9HB71 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Calcyclin-binding protein (CACYBP) is a ~26 kDa protein. May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HL60 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate
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