Anti-CASP1/Caspase-1 Polyclonal Antibody (HB991014)

Name: Anti-CASP1/Caspase-1 Polyclonal Antibody
Brand: abinScience
SKU: HB991014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB991014

설명

Anti-CASP1/Caspase-1 Polyclonal Antibody (HB991014) is a rabbit polyclonal antibody detecting Caspase-1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CASP1/Caspase-1 (Asn120-Asp297).

타겟

IL1BC, Interleukin-1 beta-converting enzyme, p45, Caspase-1, IL-1BC, IL-1 beta-converting enzyme, CASP1, IL1BCE, ICE, CASP-1, Interleukin-1 beta convertase

정제

Purified by antigen affinity column.

등록번호

P29466

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Caspase-1 (CASP1) is a ~45 kDa protein. Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part.

1. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478

2. Feng, Q. et al. (2004) Genomics 84, 587-91. PMID: 15498465

3. Thornberry, NA. et al. (1992) Nature 356, 768-74. PMID: 1574116

4. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003

5. Ball, DP. et al. (2020) Life science alliance 3. PMID: 32051255

6. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

7. Alnemri, ES. et al. (1995) The Journal of biological chemistry 270, 4312-7. PMID: 7876192

8. Akita, K. et al. (1997) The Journal of biological chemistry 272, 26595-603. PMID: 9334240

9. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CASP8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate

참고 문헌

데이터시트

Anti-CASP1/Caspase-1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CASP1/Caspase-1 Polyclonal Antibody (HB991014)

개요

이미지

참고 문헌

데이터시트

SDS

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