Anti-CASP4 Polyclonal Antibody (HW474014)

Name: Anti-CASP4 Polyclonal Antibody
Brand: abinScience
SKU: HW474014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HW474014

설명

Anti-CASP4 Polyclonal Antibody (HW474014) is a rabbit polyclonal antibody detecting CASP4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CASP4 (Asn78-Glu265).

타겟

Mih1, ICH2, CASP-4, ICE and Ced-3 homolog 2, CASP4, Protease TX, Caspase-4, ICE(rel)-II, ICH-2

정제

Purified by antigen affinity column.

등록번호

P49662

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Caspase-4 (CASP4) is a ~43 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor.

1. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034

2. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003

3. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412

4. Li, Z. et al. (2021) Nature 599, 290-295. PMID: 34671164

5. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519

6. Devant, P. et al. (2023) Nature 624, 451-459. PMID: 37993712

7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

8. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

10. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-CASP4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CASP4 Polyclonal Antibody (HW474014)

개요

이미지

참고 문헌

데이터시트

SDS