Anti-CASP8 Polyclonal Antibody (HC266014)

Name: Anti-CASP8 Polyclonal Antibody
Brand: abinScience
SKU: HC266014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC266014

설명

Anti-CASP8 Polyclonal Antibody (HC266014) is a rabbit polyclonal antibody detecting CASP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CASP8 (Ser217-Asp479).

타겟

CAP4, MCH5, FADD-homologous ICE/ced-3-like protease, FLICE, Apoptotic cysteine protease, MORT1-associated ced-3 homolog, MACH, FADD-like ICE, ICE-like apoptotic protease 5, CASP8, CASP-8, Caspase-8, Apoptotic protease Mch-5

정제

Purified by antigen affinity column.

등록번호

Q14790

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

2. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

3. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

4. Boldin, MP. et al. (1996) Cell 85, 803-15. PMID: 8681376

5. Muzio, M. et al. (1996) Cell 85, 817-27. PMID: 8681377

6. Srinivasula, SM. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14486-91. PMID: 8962078

7. Muzio, M. et al. (1997) The Journal of biological chemistry 272, 2952-6. PMID: 9006941

8. Medema, JP. et al. (1997) The EMBO journal 16, 2794-804. PMID: 9184224

9. Denault, JB. et al. (2006) Molecular cell 23, 523-33. PMID: 16916640

10. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CASP8 Polyclonal Antibody (HC266014)

개요

이미지

참고 문헌

데이터시트

SDS