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Anti-Human Band 3 Polyclonal Antibody (HF626014)

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개요
카탈로그 번호HF626014
설명
Anti-Human Band 3 Polyclonal Antibody (HF626014) is a rabbit polyclonal antibody detecting Band 3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant CD233/SLC4A1 (Ala35-Ser290).
타겟 AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, CD233, DI, EPB3, SLC4A1, Solute carrier family 4 member 1
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Purified by antigen affinity column.
등록번호 P02730
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Band 3 anion transport protein (CD233/SLC4A1) is a ~101 kDa protein. Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

1. Bruce, LJ. et al. (2000) The Biochemical journal 350 Pt 1, 41-51. PMID: 10926824
2. Rungroj, N. et al. (2004) The Journal of biological chemistry 279, 13833-8. PMID: 14734552
3. Schofield, AE. et al. (1992) Journal of molecular biology 223, 949-58. PMID: 1538405
4. Bruce, LJ. et al. (2005) Nature genetics 37, 1258-63. PMID: 16227998
5. Ungsupravate, D. et al. (2010) Molecular membrane biology 27, 92-103. PMID: 20151848
6. Shnitsar, V. et al. (2013) The Journal of biological chemistry 288, 33848-33860. PMID: 24121512
7. Genetet, S. et al. (2017) Scientific reports 7, 46170. PMID: 28387307
8. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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