Anti-Human IL-10 R alpha Polyclonal Antibody (HC614014)

Name: Anti-Human IL-10 R alpha Polyclonal Antibody
Brand: abinScience
SKU: HC614014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC614014

설명

Anti-CDw210a/IL10RA Polyclonal Antibody (HC614014) is a rabbit polyclonal antibody detecting CDw210a in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CDw210a/IL10RA (Leu132-Asn235).

타겟

IL-10 receptor subunit alpha, IL-10R1, Interleukin-10 receptor subunit alpha, Interleukin-10 receptor subunit 1, CD210, IL10RA, CDw210a, IL-10R subunit alpha, IL-10R subunit 1, IL10R, IL-10RA

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

Q13651

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Interleukin-10 receptor subunit alpha (CDw210a/IL10RA) is a ~63 kDa protein. Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes.

1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608

2. Usacheva, A. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 1302-8. PMID: 12133952

3. Shi, J. et al. (2016) Cell death & disease 7, e2133. PMID: 26962683

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human IL-10 R alpha Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human IL-10 R alpha Polyclonal Antibody (HC614014)

개요

이미지

참고 문헌

데이터시트

SDS

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