Anti-CFL1 Polyclonal Antibody (HB213014)

Name: Anti-CFL1 Polyclonal Antibody
Brand: abinScience
SKU: HB213014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HB213014

설명

Anti-CFL1 Polyclonal Antibody (HB213014) is a rabbit polyclonal antibody detecting CFL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CFL1 (Ser3-Leu161).

타겟

Cofilin, non-muscle isoform, CFL1, p18, 18 kDa phosphoprotein, CFL, Cofilin-1

정제

Purified by antigen affinity column.

등록번호

P23528

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cofilin-1 (CFL1) is a ~18 kDa protein. Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

1. Yeoh, S. et al. (2002) Journal of molecular biology 315, 911-25. PMID: 11812157

2. Gohla, A. et al. (2005) Nature cell biology 7, 21-9. PMID: 15580268

3. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987

4. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CFL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: H1299 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

참고 문헌

데이터시트

Anti-CFL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CFL1 Polyclonal Antibody (HB213014)

개요

이미지

참고 문헌

데이터시트

SDS