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Anti-CFP Polyclonal Antibody (HB792014)

Anti-CFP Polyclonal Antibody
Anti-CFP Polyclonal Antibody
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  • 개요

  • 이미지

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개요
카탈로그 번호HB792014
설명
Anti-CFP Polyclonal Antibody (HB792014) is a rabbit polyclonal antibody detecting CFP in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human CFP (Gln65-Cys190).
타겟 PFC, CFP, Properdin, Complement factor P
정제 Purified by antigen affinity column.
등록번호 P27918
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Properdin (CFP) is a ~51 kDa protein. A positive regulator of the alternate pathway (AP) of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).

1. Hourcade, DE. (2006) The Journal of biological chemistry 281, 2128-32. PMID: 16301317
2. Ferreira, VP. et al. (2010) Immunobiology 215, 932-40. PMID: 20382442
3. Pedersen, DV. et al. (2017) The EMBO journal 36, 1084-1099. PMID: 28264884
4. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277
5. Pedersen, DV. et al. (2019) Frontiers in immunology 10, 2007. PMID: 31507604
참고사항 For research use only.
이미지
  • Anti-CFP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CFP polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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