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Anti-CHPV N/Nucleoprotein Polyclonal Antibody (VK773014)

Anti-CHPV N/Nucleoprotein Polyclonal Antibody
Anti-CHPV N/Nucleoprotein Polyclonal Antibody
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  • 개요

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개요
카탈로그 번호VK773014
설명
Anti-CHPV N/Nucleoprotein Polyclonal Antibody (VK773014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Chandipura virus (CHPV)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Chandipura virus (CHPV) N/Nucleoprotein (Met1-Ala422).
타겟 Nucleoprotein, NP, Nucleocapsid protein, Protein N, N
정제 Purified by antigen affinity column.
등록번호 P11211
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Nucleoprotein is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

참고사항 For research use only.
이미지
  • Anti-CHPV N/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-N-CHPV polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant CHPV N/Nucleoprotein (Catalog No: VK773012)

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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