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| 카탈로그 번호 | HV315014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human CRYL1 (Met1-Gln319). | ||||||||
| 타겟 | Gul3DH, CRY, L-gulonate 3-dehydrogenase, Lambda-crystallin homolog, CRYL1 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q9Y2S2 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Lambda-crystallin homolog (CRYL1) is a ~35 kDa protein. Catalyzes the conversion of L-gulonate to 3-dehydro-L-gulonate. It also exhibits low dehydrogenase activity toward L-3-hydroxybutyrate (HBA) and L-threonate. Utilizes NAD as the sole cosubstrate and shows no activity with NADP. 1. Ishikura, S. et al. (2005) Journal of biochemistry 137, 303-14. PMID: 15809331 2. Malatesta, M. et al. (2025) Proceedings of the National Academy of Sciences of the United States of America 122, e2506597122. PMID: 40737316 | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CRYL1 (HV315014) at a concentration of 23.75 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CRYL1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-CRYL1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MOuse liver lysate
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