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Anti-CTSG Polyclonal Antibody (HY284014)

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개요
카탈로그 번호HY284014
설명
Anti-CTSG Polyclonal Antibody (HY284014) is a rabbit polyclonal antibody detecting CTSG in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human CTSG (Ile21-Leu255).
타겟 Cathepsin G, CG, CTSG
정제 Purified by antigen affinity column.
등록번호 P08311
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Cathepsin G (CTSG) is a ~28 kDa protein. Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor.

1. Thorpe, M. et al. (2018) PloS one 13, e0195077. PMID: 29652924
2. Avril, LE. et al. (1994) FEBS letters 345, 81-6. PMID: 8194606
3. Bangalore, N. et al. (1990) The Journal of biological chemistry 265, 13584-8. PMID: 2116408
4. Alford, CE. et al. (1990) Journal of general microbiology 136, 997-100. PMID: 2117044
5. Sambrano, GR. et al. (2000) The Journal of biological chemistry 275, 6819-23. PMID: 10702240
6. Selak, MA. et al. (1988) The Biochemical journal 251, 293-9. PMID: 3390156
7. Hase-Yamazaki, T. et al. (1995) Cellular immunology 160, 24-32. PMID: 7842483
8. Yamazaki, T. et al. (1997) Journal of leukocyte biology 61, 73-9. PMID: 9000539
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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